Our Projects
Using 3D organoids derived from patient samples (PDOs) or esophageal cancer cell lines, the Sachdeva Lab is studying the responses of esophageal epithelial and stromal cells to metabolic stress, and the way in which these stress response pathways contribute to malignant transformation.
Basic and translational projects in the lab focus on the following key areas:
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Investigating metabolic shifts in esophageal non-transformed epithelial and cancer cells in response to acid- and bile-induced stresses
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Determining the role of stromal-derived cytokines in promoting early esophageal cancer development
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Developing PDO libraries from normal esophageal epithelium, Barrett’s esophagus and esophageal adenocarcinoma and squamous cell carcinoma from primary patient biopsy samples
Our experiments use 3D organoid cultures (here, shown embedded in Matrigel) to study tumor biology and microenvironment interactions in esophageal cancer.